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OBJECTIVE：To study the prepar ation technology of gastric floating tablets of Schisandra chinensis total lignans （SCTL），and evaluate the quality of prepared tablets. METHODS ：Based on single factor test ，the orthogonal experiment was conducted to optimize the formulation of SCTL gastric floating tablets with the contents of hydroxypropylmethylcellulose （HPMC） K15M，NaHCO3 and microcrystalline cellulose as the factors ，using starting time ，holding time and cumulative release rate of gastric floating tablets as evaluation indexes. The properties ，weight difference ，floatability and accumulative release rate of the prepared SCTL gastric floating tablets were determined. The gastric floating tablets were qualitatively identified by TLC ，and the contents of schisandrin A and total lignans were determined by HPLC and UV spectrophotometry. RESULTS ：The optimal formulation of SCTL gastric floating tablets was made up of 23％ SCTL extract ，20％ HPMC K 15M，40％ microcrystalline cellulose，15％ sodium bicarbonate ，1％ octadecyl alcohol and 1％ polyvinylpyrrolidone. The results of detection of this preparation were in line with the related provisions of “0101 tablet”stated in 2015 edition of Chinese Pharmacopoeia （part Ⅳ）. TLC indicated that the chromatogram of the test sample showed the main spots of same color as the corresponding positions of the chromatogram of schizandrol A control ，Schisandra chinensis reference substance and raw material ，while the negative control has no interference. Content determination results shows that the average content of schizandrol A and total lignans in SCTL gastric floating tablets is 3.187，19.617 mg. It was preliminarily formulated that the content limitation of schizandrol A in one tablet should not be less than 2.50 mg，and the content of total lignans （calculated by schizandrol A ）should not be less than 15.50 mg. CONCLUSIONS：The preparation technology of SCTL gastric floating tablets is stable ，feasible and controllable in quality.
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Objective: To study the relativity between color and chemical composition in fruits of Schisandra chinensis by fruits' surface color value (L*, a*, and b*) and contents of active ingredients of fructus core S. chinensis). Methods: The surface chromatic value (multivariate linear regression equation of L*, a*, b* values and deoxyschizandrin, schizandrin B, schizandrol A, schinsantherin A) was established by SPSS 20.0 with mathematical statistics method, then the influence degree of the active ingredients of. S. chinensis was detected by F test. Results: The value of L* were significant negative with deoxyschizandrin (r ≥ 0.5), and other chromaticity values were very low or low-grade correlated with lignans Conclusion: The effective ingredient content of fruit of S. chinensis can be estimated by its chromatic values, and also can be used in the hierarchies of the fruit of S. chinensis, in order to provide better scientific basis of the quality of S. chinensis.
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The effectiveness of traditional Chinese medicine (TCM) against various diseases urges more low cost,speed and sensitive analytical methods for investigating the phamacology of TCM and providing a theoretical basis for clinical use.The potential of second-order calibration method was validated for the quantification of two effective ingredients of Schisandra chinensis in human plasma using spectrofluorimetry.The results obtained in the present study demonstrate the advantages of this strategy for multi-target determination in complex matrices.Although the spectra of the analytes are similar and a large number of interferences also exist,second-order calibration method could predict the accurate concentrations together with reasonable resolution of spectral profiles for analytes of interest owing to its ‘second-order advantage'.Moreover,the method presented in this work allows one to simply experimental procedure as well as reduces the use of harmful chemical solvents.
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Objective To study the quality standard of Anshen Jiannao Mixture. Methods Radix ophiopgonis, Radix et Rhizoma Salviae Miltiorrhizae, Fructus Lycii and Radix et Rhizoma Ginseng were identified by TLC. HPLC was used to determine the content of Schizandrol A on C18 (250 mm?4.6 mm, 5 ?m) ODS column. The mobile phase was consisted of methanol-water (55∶45). The flow rate was 1.0 mL/min. The detection wavelength was at 250 nm. Results The characteristic spot of Radix et Rhizoma Salviae Miltiorrhizae, Fructus Lycii and Radix et Rhizoma Ginseng could be easily examined by TLC. The calibration curve of Schizandrol A was linear in the range of 0.121 8～1.218 ?g, r=0.999 9. The precision RSD was 0.22% (n=6). The reproducibility RSD was 1.1% (n=6). The average recovery rate of Schizandrol A was 97.34% (RSD=1.7%, n =6). Conclusions The method is easy, sensitive and specific with good reproducibility. It can be used to control the quality of Anshen Jiannao Mixture.
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Objective To establish a HPLC determination method for the effective constituents extracted from Fructus Schisandrae Chinensis by low-temperature water extraction.Methods The assay was conducted on Kromasil C18 column(250 mm? 4.6 mm,5 ? m)using a gradient elution with acetonitrile-water as mobile phase.The flow rate was set at 1.0 mL/min,column temperature was kept at 30 ℃,and the detection wavelength was at 240 nm.Results The linear range for schizandrol A was 5.0～ 200.0 ? g/mL(r=0.999 9),and the average recovery was 101.27 % with RSD being 1.97 %(n=6).Conclusion The method is simple,reliable and reproducible for the determination of schizandrol A.It can be used for quality control of preparations including Fructus Schisandrae Chinensis.
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AIM: To explore the biopharmaceutical character of the combination of Fructus Schisandrae and Radix Salvia Miltiorrhizae. METHODS: Mice were divided into four group according to schizandrol A, Schizandrin B, Fructus Schisandrae and combination with Radix salvia Miltiorrhizae and then perfused. Mouse's bood plasma was collected in sequence, and the test solusion was prepared by adding amine sulfate and the mixture of acetonitrile methanol (4∶1). Its contents were measure by HPLC in the aid of SIP and 3p97 program. RESULTS: Metabolic rate in mouse of schizandrol A and schizandrin B from combination is apparently lower than that of non combination. CONCLUSION: Chinese Traditional medicinal combination has its biopharmaceutical value.
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AIM: To establish a method for determination of schizandrol A in Mian'er'an Dripping Pills (Semen Ziziphi Spinosae, Fructus Schisandrae, etc.). METHODS: HPLC was used with hypersil C 18 column (250mm?4.6mm; 5?m), methanol water (65∶35) as a mobile phase and detection wavelength at 254nm. The flow rate was 1.0mL?min -1 and at room temperature. RESULTS: The calibration curve was linear (r= 0.9999). The average recovery was 100.2% and RSD was 2.9%, respectively. CONCLUSION: The method is simple, quick and specific, and provides a quality control for the dripping pills.
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AIM: To develop a method to determine the content of Tanshinone Ⅱ A and Schizandrol A in Anshenbuxin Pill(Radix Salviae Miltiorrhizae, Fructus Schisandrae Chinensis, Rhizoma Acori Tatarinowii,etc.). METHODS: HPLC was used to determine the content of Tanshinone Ⅱ A and Schizandrol A in Anshenbuxin Pill. The separation was performed on C 18 column with methanol water (80∶20) for Tanshinone Ⅱ A and methanol water(50∶50) for Schizandrol A, respectively. The detection wavelength was at 270nm for Tanshinone Ⅱ A and 250nm for Schizandrol A, respectively. RESULTS: The average recovery of Tanshinone Ⅱ A was 98.54%( RSD =1.75%, n =6) and the average recovery of Schizandrol A was 99.05%( RSD =1.77%, n =6). CONCLUSION: This method is simple, accurate with good reproducibility and can be used to control the quality of Anshenbuxin Pill.
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AIM:To research the stability of Compound Wurenchun Soft Capsule in 18 months. METHODS:TLC and HPLC methods were used.Schizandrol A was separated on a ODS-C_(18) column(Lichrospher ODS-C_(18),(4.6 mm?250 mm),) using methyl alcohol-water (65∶35) as the mobile phase,detected at 250 nm.The velocity of flow was 1.0 mL/min.Paeoniflorin was separated on a ODS-C column(Lichrospher ODS-C_(18),4.6 mm?250 mm),using acetonitrile-water-phophoric acid(15∶85) as the mobile phase,detected at 230 nm,the velocity of flow was 1.0 mL/min. RESULTS:There was a good linear relationship at the range of 0.04 mg/mL-0.64 mg/mL for schizandrol A(r=0.999 8).The average recovery of schizandrol A was 98.76%(n=6,RSD=1.18%).There was a good linear relationship at the range of 0.05 mg/mL-0.80 mg/mL for paeoniflorin(r=0.999 9).The average recovery of paeoniflorin was 97.93%(n=5,RSD=1.68%). CONCLUSION:In 18 months,the quality of Compound Wurenchun Soft Capsule is steady.